# FUNCTIONAL COMPARTMENTALIZATION OF NEURONS AND GLIA

> **NIH NIH R01** · EMORY UNIVERSITY · 1992 · $153,219

## Abstract

The objectives are to continue our studies to:  1) identify, isolate and
characterize intracellular pools of membrane bound glycoconjugates
undergoing fast axonal transport, 2) to characterize different functional
compartments of the neuronal cytoskeleton related particularly to
phosphorylation of component and associated polypeptides of cytoskeletal
organelles.  Immunocytochemical analysis will be used to study the
distribution of glycoprotein antigens in cerebellum whose biochemical
properties mimic the cytochemical properties of specialized smooth membrane
systems in Purkinje cell axons and dendrites.  We will use specific
radioisotope labeling together with pharmacological manipulations of
oligosaccharide processing and axonal transport to identify the origin and
fate of newly synthesized glycoconjugates destined for fast axonal
transport in retinal photoreceptor and ganglion cells.  Enzyme-linked
cytochemical, autoradiographic and biochemical analysis will be used to
begin to characterize oligosaccharide chains of transported glycoconjugates
compared to those that have reached the presynaptic terminal of retinal
neurons.  We will use immunocytochemical and immuno-blot analysis of tissue
sections, cultured neurons and isolated fractions of cytoskeletal
organelles to study the intracellular compartmentalization of the
phospholipid/Ca2+-dependent protein kinase (PKC) and its substrates in
axons.  Endogenous and exogenous PKC will be used in in vitro
phosphorylation experiments for comparison with in vivo labeling patterns
to elucidate specific substrates for the enzyme among the cytoskeletal
polypeptides.  Retinal tissue slices and cultures of retinal and
sympathetic ganglion neurons will be used to ask the question whether or
not PKC translocates from the cytosol to the plasma membrane of neurons in
response to physiological stimulation or pharmacological activation of
PKC.  We will use non-hydrolyzable analogs of ATP to probe microtubule
deficent axons of Myxicola infundibulum for the presence of "kinesin" type
translocator molecules.  Kinesin preparations from rat brain will be used
to test for the presence of axonal polypeptides associated with the
cytoskeleton, particularly the tau proteins and PKC.

## Key facts

- **NIH application ID:** 3123107
- **Project number:** 9R01AG011123-12
- **Recipient organization:** EMORY UNIVERSITY
- **SAM.gov UEI:** S352L5PJLMP8
- **Principal Investigator:** JOHN G WOOD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $153,219
- **Award type:** 9
- **Project period:** 1992-06-15 → 1997-05-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3123107

## Citation

> US National Institutes of Health, RePORTER application 3123107, FUNCTIONAL COMPARTMENTALIZATION OF NEURONS AND GLIA (9R01AG011123-12). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3123107. Licensed CC0.

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