# BIOLOGY OF EXTRACELLULAR BETA-AMYLOID PROTEIN PRECURSOR

> **NIH NIH R01** · SALK INSTITUTE FOR BIOLOGICAL STUDIES · 1993 · $182,961

## Abstract

Recent evidence shows that the amyloid beta protein precursor (ABPP) is
secreted from cells and can be incorporated into extracellular matrix
(ECM).  ECM is a major component of the developing nervous system and it is
also juxtapositioned between the pre and post synaptic cells of CNS
synapses.  It is possible that Alzheimer's plaques and associated cognitive
losses arise from the aberrant metabolism of ABPP in ECM.  Alternatively,
altered expression of ABPP in Alzheimer's patients may change the synthesis
and breakdown of other ECM components.  To test these alternatives, we
propose to examine the effect of ABPP on the metabolism of extracellular
matrix deposited by clonal nerve cell lines as well as the effect of
proteases secreted by CNS cells on the breakdown of ABPP in ECM.  These
studies will use rodent nerve cell lines which synthesize ABPP as well as a
rat cell , which makes no ABPP of its own, and a derivative of this line
transfected with human ABPP.  Specifically, the breakdown of metabolically
labeled ECM deposited by neuronal cell lines will be induced by proteases,
and the effect of the forms of ABPP with and without protease inhibitor on
this process examined.  In addition, the effects of ABPP on its own
breakdown within the ECM will be examined.  These experiments should either
support or rule out the possibility that ABPP is involved in the metabolism
of CNS matrix, and shed some light upon a role of this process in
Alzheimer's disease.  In addition, experiments are proposed which will
examine the cell-cell and cell-substratum adhesion inducing properties of
ABPP.  These adhesive interactions will be studied in the context of their
role in cell division, neurite outgrowth, and neurotrophic effects.

## Key facts

- **NIH application ID:** 3123329
- **Project number:** 5R01AG011383-02
- **Recipient organization:** SALK INSTITUTE FOR BIOLOGICAL STUDIES
- **SAM.gov UEI:** NNJ6BMBTFGN5
- **Principal Investigator:** DAVID R SCHUBERT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $182,961
- **Award type:** 5
- **Project period:** 1992-08-01 → 1995-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3123329

## Citation

> US National Institutes of Health, RePORTER application 3123329, BIOLOGY OF EXTRACELLULAR BETA-AMYLOID PROTEIN PRECURSOR (5R01AG011383-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3123329. Licensed CC0.

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