# DNA ENZYMES IN AGING IN DIVIDING & NONDIVIDING CELLS

> **NIH NIH R01** · UNIVERSITY OF SOUTHERN CALIFORNIA · 1992 · $320,938

## Abstract

This proposal focuses on developing an in vitro model to study how DNA
replication and repair enzymes act on defined site-directed lesions in
DNA.  Little is known concerning the repertoire of DNA metabolizing
enzymes available in neurons and astrocytes, and how these enzymes respond
to DNA damage and vary with age.  Two extremely sensitive assays, based on
polyacrylamide gel electrophoresis, will be used to study the behavior of
DNA synthetic and repair enzymes with three classes of DNA lesions, an
abasic (apurinic/apyrimidinic) lesion, alkylated guanine and thymine
nucleotide analogues, and 8-oh guanine, a lesion resulting from oxidative
damage.  The first assay will be used to identify and characterize
replication and repair DNA polymerases, using extracts from liver and
brian in young and old animals, and in dividing and nondividing cells
grown in culture.  There is a paucity of data on the ability of the
various DNA polymerases to copy and bypass base and sugar modified DNA
lesions.  The second assay will be used to identify enzymes, such as
glycosylases, methyltransferases, endo- and exonucleases, and ligases,
involved in lesion repair.  We intend to assay crude extracts of brain and
liver cells for activities that are induced in response to DNA damage, and
to purify and characterize these enzymes.  We will determine if animal
cells exhibit an induced "error-prone" response to DNA damage analogous to
the SOS response in Escherichia coli and other enterobacteria.

## Key facts

- **NIH application ID:** 3123349
- **Project number:** 1R01AG011398-01
- **Recipient organization:** UNIVERSITY OF SOUTHERN CALIFORNIA
- **SAM.gov UEI:** G88KLJR3KYT5
- **Principal Investigator:** MYRON GOODMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $320,938
- **Award type:** 1
- **Project period:** 1992-08-01 → 1997-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3123349

## Citation

> US National Institutes of Health, RePORTER application 3123349, DNA ENZYMES IN AGING IN DIVIDING & NONDIVIDING CELLS (1R01AG011398-01). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3123349. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*