# IMMUNOLOGICAL MECHANISM OF ANAPHYLAXIS

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1988 · $143,021

## Abstract

It will be attempted to produce monoclonal antibodies against allotype 7a
and 7b, the two most important allotypes of murine IgE, the antibody
responsible for anaphylactic reactions.  Allotype 7b does not crossreact
with any other allotype T antibodies and allotype 7a crossreacts with all
the other allotype 7 antibodies except with allotype 7b.  These two
monoclonal antibodies (anti-7a and anti-7b) would be of great importance
for research in experimental allergic reactions.  For the production of
these monoclonal antibodies the hybridoma technique will be used.  Spleen
cells from inbred mice immunized against allotype 7a or 7b and producing
anti-7a or anti-7b antibodies will be fused with a nonproducing myeloma
cell and the hybridoma cells obtained will be checked for the production of
anti-7a or anti-7b monoclonal antibodies, using as test passive
hemagglutination of sheep red blood cells coated with 7a or 7b IgE
antibodies.

T cells from inbred mice primed with well defined immunogenic antigens,
will be cloned, using a standard method of T cell cloning.  Long term
cultures will be established from those cell lines which have specificity
for the antigen demonstrated by radiolabeled thymidine incorporation.
These T cell lines will be then tested in vitro for antigen specific help
using primed B cells from inbred mice syngeneic to the mice from which the
T cells were cloned.  The B cells will be from mice primed with
hapten-carrier protein complex, but the carrier protein will be different
from that used to prime the T cells.  In the in vitro tests the same hapten
that was used for priming the B cells will be coupled to the antigen used
for the T cells.  In this way it will be possible to ascertain antigen
specific help by the T cells and the role of these T cells in production of
different murine isotypes, especially IgE antibody.

T cells from SJA9 mice taken from mice infected with Nippostrongylus
brasiliensis will be cloned on feeder cells from Nippostrongylus infected
mice.  It will be attempted to clone by this procedure the T cell which
brings about the switch for IgE production in this strain.  The cloned T
cells will be tested in SJA9 mice in vivo and also with SJA9 spleen cells
from primed mice in vitro.

## Key facts

- **NIH application ID:** 3124121
- **Project number:** 5R01AI003075-29
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** ZOLTAN OVARY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $143,021
- **Award type:** 5
- **Project period:** 1974-12-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124121

## Citation

> US National Institutes of Health, RePORTER application 3124121, IMMUNOLOGICAL MECHANISM OF ANAPHYLAXIS (5R01AI003075-29). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124121. Licensed CC0.

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