# VIRAL INTERFERENCE IN CARRIER CULTURES

> **NIH NIH R01** · HAHNEMANN UNIVERSITY · 1987 · $144,344

## Abstract

Our studies are continuing to focus on defining the early events in
coxsackievirus infections.  We have provided evidence, by receptor
competition, that many prototype picornaviruses sort into the subgroups in
which they were classified originally by disease production and
histopathology.  We propose to evaluate the dual functions of receptors to
attach and eclipse coxsackeiviruses and our multicomponent receptor
hypothesis.  We are continuing our efforts to purify and characterize the
membrane receptor (R1) which binds the prototype group B coxsackievirused
to HeLa cells.  This receptor will be compared to a second receptor (R2)
which will be purified from RD cells and to a third receptor (R3) obtained
from human O erythrocytes.  Monoclonal antibodies to the different
receptors also will be prepared and utilized as probes to help characterize
and isolate the receptors.  RD cells select hemagglutinating (HA+) group B
coxsackievirus variants (host range mutants) from HA- populations.  Since
HA+ variants produce a modified histopathology and minimal disease in mice,
it will be important to determine the role of the different receptors in
virulence.  Our studies of differentiating myogenic rat cells (L8) in
culture have shown that coxsackieviruses of group A and B3 will bind to
separate receptors on non-fusing cells, but are not eclipsed until the
cells are capable of fusion.  Plans are made to determine whether eclipse
is mediated by:  (1) accessory factors or (2) a rearrangement of receptors
during differentiation.  It is anticipated that the results will determine
the adegree of homology between receptors for the group B coxsackeiviruses
and determine those early events in virus-cell interactions which may be
associated with avirulence or virulence of selected virus strains.

## Key facts

- **NIH application ID:** 3124144
- **Project number:** 5R01AI003771-26
- **Recipient organization:** HAHNEMANN UNIVERSITY
- **Principal Investigator:** RICHARD L CROWELL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $144,344
- **Award type:** 5
- **Project period:** 1975-05-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124144

## Citation

> US National Institutes of Health, RePORTER application 3124144, VIRAL INTERFERENCE IN CARRIER CULTURES (5R01AI003771-26). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124144. Licensed CC0.

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