# IRON METABOLISM OF MICROORGANISMS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA BERKELEY · 1987 · $120,558

## Abstract

The high affinity, siderophore mediated iron assimilation systems of
prokaryotic and eukaryotic microorganisms will be investigated by
application of the standard techniques of molecular genetics.  The
biosynthesis and transport components of the cloned, plasmid-derived,
aerobactin system of hospital isolates of Escherichia coli will be
defined.  Definitive proof for the pathway from lysine to N
omega-hydroxylysine to aerobactin will be sought and the enzymes
characterized.  The nature of the transcriptional regulation of this system
by iron will be studied to determine if the message accumulated at low iron
results from iron-repressor binding to the promoter-operator or,
alternatively, from altered rates of turnover of the mRNA.  Preliminary
experiments will be aimed at cloning all or part of the rhodotorulic acid
siderophore system of the basidiomycetous yeast Rhodotorula pilimanae.

## Key facts

- **NIH application ID:** 3124176
- **Project number:** 5R01AI004156-26
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **SAM.gov UEI:** GS3YEVSS12N6
- **Principal Investigator:** JOHN B NEILANDS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $120,558
- **Award type:** 5
- **Project period:** 1976-05-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124176

## Citation

> US National Institutes of Health, RePORTER application 3124176, IRON METABOLISM OF MICROORGANISMS (5R01AI004156-26). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124176. Licensed CC0.

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