# BIOCHEMICAL BASIS OF DELAYED-TYPE HYPERSENSITIVITY

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1992 · $323,473

## Abstract

The aims of this project remain to provide an understanding in molecular
terms of the roles of lymphocytes and macrophages in cell-mediated
immunity.  The approach will place major emphasis on cloned cell lines
expressing specific macrophage or lymphocyte functions, and the use of
genetic techniques to select of variants in specific functions.  A major
effort will be made to pursue these aims using human cell lines studied
within the context of a unique human disease model, leprosy.

To better understand the immunoregulatory changes occurring in lepromatous
leprosy patients who are specifically unresponsive to lepromin, we propose
to establish T-helper and T-suppressor cell lines, to define the
requirements for in vitro sensitization of normal T cells to mycobacterial
antigens, and those for in vitro induction of T-suppressor cells, and
analyzing the specificity and mode of action of the T-suppressor cells on
vitro sensitization, particularly production of suppressor factors.

To better understand bactericidal effector mechanisms, we propose to
develop human monocyte/macrophage cell lines, study macrophage cytocidal
mechanisms through the use of variants in oxygen metabolism, develop a
cell-free system for in vitro production of O2-, characterize the critical
membrane enzymes, and define the mechanisms of priming and triggering for
an oxidative burst.  Attempts will be made to reconstitute these lines with
enzymes and drugs to enhance intracellular cytocidal activity.  Finally, a
variety of variants in macrophage cell lines defective in interferon
responsiveness, cytoskeletal functions and lysosomal enzymes will be
studied to define critical cellular requirements for antigen presentation
and activation for intracellular killing.

## Key facts

- **NIH application ID:** 3124340
- **Project number:** 5R01AI007118-27
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** BARRY R. BLOOM
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $323,473
- **Award type:** 5
- **Project period:** 1979-01-01 → 1993-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124340

## Citation

> US National Institutes of Health, RePORTER application 3124340, BIOCHEMICAL BASIS OF DELAYED-TYPE HYPERSENSITIVITY (5R01AI007118-27). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124340. Licensed CC0.

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