# IMMUNOLOGICAL STUDIES WITH SYNTHETIC POLYPEPTIDES

> **NIH NIH R01** · THOMAS JEFFERSON UNIVERSITY · 1987 · $196,236

## Abstract

The research will continue to employ synthetic polymers of amino acids (T
cell dependent and major histocompatibility-complex linked) to study
aspects of genetic control of the immune response (lr) in mice.  Wild mice
(B10.W) having known la antigens, and H-2bm12 mice with mutations lA will
be immunized with H-2b linked immunogens to determine the association of lr
and known la antigens; i.e. lr=la?  Anti-lD against the polymers GLPhe,
GPhe, GAPhe, GLA40 and some sequential polymers will be studied to
determine a) whether the anti polymer idiotypic determinants (VH region?)
from mice within a murine strain as well as with different strains might be
conserved and if so the extent of it and b) the possible importance of the
association of the lD determinants in anti-GPhe with H2q.  The biological
activities of the various anti-lD as they effect both B cell i.e. PFC
responses, and T cell functions i.e. proliferative responses: will be
investigated.  The anti-lD will be used to determine the nature of the ID
determinants on suppressor, or helper T cells: or "factors" from responder
and non-responder mice.  The characterization of antigen specific T cell
receptors (GLA40 system) will continue and will include determining the
possible association of lD and la determinants with "the receptor."  Our
present success in the production of rabbit anti-lD sera against GLA40 and
the production of hybridomas (monoclonal) against GLA40 are important for
receptor work.  In order to obtain significant amounts of T cells for
isolation of receptors, we are developing techniques for T cell cloning and
hybridization. The following systems of indicated H-2 will be studied to
determine the number of unique T cell lines that may be produced and
separated, and the antigen presenting cell haplotype specificities that may
be involved.  The l-GLPhe9:a) Fl(H-2f x H-2s) responding to GLPhe9 and GLT,
b) Fl(H-2d x H-2s) responding to GLPhe9, GLT and GPhe, c) Fl(H-2d x H-2q)
responding to GLPhe9, GLT and GPhe, d) H-2q responding to GLT and GPhe.
The 2-GLT15: Fl(H-2f x H-2s) responding to GLT15 and GLPhe.  The 3-GPhe:
Fl(H-2d x H-2s).  Sufficient amounts of antibody are being prepared against
some of the sequential polymers so that anti-lD sera can be raised and the
lD determinants characterized and compared within a single respnding
haplotype congenic mice or from 2 different responding haplotypes.

## Key facts

- **NIH application ID:** 3124392
- **Project number:** 5R01AI007825-21
- **Recipient organization:** THOMAS JEFFERSON UNIVERSITY
- **SAM.gov UEI:** R8JEVL4ULGB7
- **Principal Investigator:** PAUL H MAURER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $196,236
- **Award type:** 5
- **Project period:** 1978-01-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124392

## Citation

> US National Institutes of Health, RePORTER application 3124392, IMMUNOLOGICAL STUDIES WITH SYNTHETIC POLYPEPTIDES (5R01AI007825-21). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124392. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*