# GENETICS OF LAMBDA PROPHAGE - BIOTIN OPERON

> **NIH NIH R01** · STANFORD UNIVERSITY · 1987 · $303,696

## Abstract

The DNA base sequence of the pI promoter of the lambda-related phage 21 will be
determined.  We have already sequenced pI from phage 434.  Phage 21 is of
interest because, unlike 434, it has a different insertion site than lambda.
However, its pI promoter responds to the cII protein.  Mutations in the pI
promoter of lambda will be identified and sequenced.
The structural gene for biotin sulfoxide reductase (bisC) will be cloned on a
multi-copy plasmid.  This should provide us with sufficient bisC protein to
obtain a pure product, as well as facilitating genetic analysis.  Insertions of
Mu Ap 1ac in bisC will be selected so that the regulation, if any, of bisC can
be examined.  We have found that this enzyme uses the same molybdenum cofactor
(product of genes ch1A, ch1B, ch1E, ch1G) as nitrate reductase.  The relation of
those genes to bisC regulation will be tested.

The birA gene, structural gene for the bifunctional protein
holoenzyme-synthetase biotin-repressor, already cloned as a 2.1 kb segment, will
be sequenced in wild type and mutant form.

## Key facts

- **NIH application ID:** 3124422
- **Project number:** 5R01AI008573-20
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** ALLAN M CAMPBELL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $303,696
- **Award type:** 5
- **Project period:** 1978-06-01 → 1988-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124422

## Citation

> US National Institutes of Health, RePORTER application 3124422, GENETICS OF LAMBDA PROPHAGE - BIOTIN OPERON (5R01AI008573-20). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124422. Licensed CC0.

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