# GENETICS OF LAMBDA PROPHAGE--BIOTIN OPERON

> **NIH NIH R01** · STANFORD UNIVERSITY · 1990 · $275,345

## Abstract

The long term goals are to understand how viruses interact with
chromosomes and how genes for biosynthesis of proteins needed in
small amounts are regulated.  The health relatedness of these
studies on prokaryotic model systems lies in the elucidation of
basic mechanisms and in the perspective shed on natural biology
of disease-causing viruses by the common features of bacterial
and animal virology.  The DNA of bacteriophage lambda upstream
of the integrase operon will be examined for effects on
integration and excision of two elements:  a terminator-like
sequence tI' that abuts the pI promoter and an open reading frame
(ORF-55) upstream from it.  Phage constructed to eliminate tI'
will be examined for the effect of cII protein in the presence of
transcription from pL, to test the hypothesis that tI' functions in
the initial transition from the uncommitted to the lysogenic state.
The ORF-55 gene expressed from a plasmid will be tested for
complementation of the excisionase defect of the lambda crg
phage, which has an IS2 insertion in ORF-55.  Lambda crg
prophages in inverted orientation will be tested for the ability to
generate duplications by recombination with the defective gsr'
prophage, and the defective prophages of E. coli strains of the
ECOR collection will be compared.  The properties of purified E.
coli biotin sulfoxide reductase and the molecular basis for a newly
evolved BDS reductase activity will be determined.

## Key facts

- **NIH application ID:** 3124424
- **Project number:** 5R01AI008573-23
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** ALLAN M CAMPBELL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $275,345
- **Award type:** 5
- **Project period:** 1978-06-01 → 1993-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124424

## Citation

> US National Institutes of Health, RePORTER application 3124424, GENETICS OF LAMBDA PROPHAGE--BIOTIN OPERON (5R01AI008573-23). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124424. Licensed CC0.

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