# BIOSYNTHESIS OF LIPOPOLYSACCHARIDE

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 1987 · $286,405

## Abstract

These studies are aimed at elucidation of 1) The pathway of biosynthesis of
lipid A in Salmonella typhimurium; and 2) the molecular mechanisms of
intermembrane translocation of lipopolysaccharide and phospholipid between inner
and outer membrane.  Genetic and biochemical approaches will be employed to
analyze the pathway of lipid A synthesis.  Conditional mutants in the pathway
will be isolated, mapped and characterized biochemically with respect to the
nature of mutant lipid A-related products formed and enzymatic reactions
involved.  Studies on translocation are aimed at identification of possible
intermediate steps in lippopolysaccharide translocation - defective mutants and
investigation of the relation of membrane topology to translocation of
phospholipid.

## Key facts

- **NIH application ID:** 3124439
- **Project number:** 5R01AI008650-19
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **SAM.gov UEI:** H6D6JMXJXDE6
- **Principal Investigator:** MARY J OSBORN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $286,405
- **Award type:** 5
- **Project period:** 1978-04-01 → 1989-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124439

## Citation

> US National Institutes of Health, RePORTER application 3124439, BIOSYNTHESIS OF LIPOPOLYSACCHARIDE (5R01AI008650-19). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124439. Licensed CC0.

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