# MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS

> **NIH NIH R01** · DUKE UNIVERSITY · 1993 · $303,876

## Abstract

The overall aim of the proposed research program is the identify the
functions of the proteins encoded by reovirus.  Six specific aims are
proposed.  The first is to isolate the native forms of all reovirus
proteins from cells infected with a powerful mammalian expression vector
into which the reovirus genome segments have been cloned.  The isolated
proteins will be examined for enzymic activities and ability to bind to RNA
and other proteins, both viral and cellular.  Second, transient expression
vectors will be used to examine the effect of reovirus proteins on DNA
replication, transcription and protein synthesis.  Genetically engineered
genome segments will be used to identify functional domains.  The
intracellular location of reovirus proteins into which reovirus RNA species
have been lipofected singly and in recombinant vaccinia virus strains
containing the various genome segments.  Third, techniques will be
developed to introduce genetically engineered genome segments into reovirus
genomes so as to permit study of the effect of alterations in functional
domains on viral phenotype.  Fourth, temperature-sensitive mutants and
anti-sense technology will be used to determine at what stage inability of
individual viral proteins to function arrests virus multiplication, thereby
providing further clues to viral protein function.  Fifth, selected SORFs
in reovirus serotype 1, 2 and 3 cell receptors will be identified in gene
libraries and sequenced in order to identify the nature of the receptors.

## Key facts

- **NIH application ID:** 3124482
- **Project number:** 5R01AI008909-25
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** Wolfgang K Joklik
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $303,876
- **Award type:** 5
- **Project period:** 1974-09-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124482

## Citation

> US National Institutes of Health, RePORTER application 3124482, MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS (5R01AI008909-25). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124482. Licensed CC0.

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