# FUNCTION OF THE KINETOPLAST IN THE HEMOFLAGELLATES

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1989 · $246,672

## Abstract

The overall goal of this project is to dissect the process of mitochondrial
biogenesis that occurs in this lower eukaryotic parasitic cell in molecular
terms, both in terms of the mitochondrial genome and the nuclear genome.
In addition we would like to understand the unusual molecular
differentiation of this mitochondrial DNA in a structural and functional
sense.  Specific aims are as follows:  1. Completion of entire sequence of
L. tarentolae maxicircle.  2. Identification of 3' and 5' ends of all
transcripts.  3. Identification of primary transcripts.  4. Isolation and
sequencing of tRNAs.  5. Transcription of specific maxicircle genes under
different physiological conditions.  6. Transcription of maxicircle genes
in life cycle of T. brucei.  7. Isolation of mitochondrial RNA polymerase.
Identification of promoters of maxicircle genes.  8. Comparative maxicircle
gene organization in different species.  9. Sequencing of 9 and 12s genes
from several species.  10. Isolation of cytochrome oxidase or FOF1 ATPase
from L. tarentolae.  11. Cloning of nuclear genes for cytochrome oxidase
subunits 4-7 of FOF1 ATPase subunits.  12. Cloning nuclear genes for
mitochondrial proteins.  13. Analysis of developmentally regulated
transcription of the BhR sequence in T. brucei.  14. Analysis of possible
minicircle transcription.

## Key facts

- **NIH application ID:** 3124506
- **Project number:** 5R01AI009102-21
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** Larry Simpson
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $246,672
- **Award type:** 5
- **Project period:** 1977-05-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124506

## Citation

> US National Institutes of Health, RePORTER application 3124506, FUNCTION OF THE KINETOPLAST IN THE HEMOFLAGELLATES (5R01AI009102-21). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124506. Licensed CC0.

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