# BIOLOGY AND BIOCHEMISTRY OF INFLUENZA VIRUSES

> **NIH NIH R01** · MOUNT SINAI SCHOOL OF MEDICINE OF CUNY · 1988 · $162,513

## Abstract

This project represents the continuation of a long-term application of
influenza virus genetics to the study of viral virulence and the
pathogenesis and epidemiology of influenza.  The model for this
investigation will be the study of naturally occurring swine influenza
virus hemagglutinin mutants and their derived reassortants.  These mutants
are dimorphic variants of current swine influenza viruses which differ
pleiotropically in antigenicity and replication characteristics in chick
embryos, MDCK cells and intact swine.  In these studies dimorphic L and H
mutants of A/NJ/11/76 H1N1 swine influenza virus and their derived
reassortant viruses will be antigenically defined further by monoclonal
antibodies which will be produced from mouse hybridomas by standard
techniques.  The reaction of L and H prototype viruses with nonspecific
inhibitors and cellular receptors will be studied in order to better
characterize the biological phenotype of these mutants and to develop
easier methods for their identification in vitro.  In collaborative studies
with others a routine assay system for the differentiation of the mutants
in swine will be developed.  Other collaborative studies now in progress
which have involved the cloning and sequencing of the hemagglutinin genes
of the L and H mutant viruses will continue to establish the nucleotide and
amino acid sequence difference which defines genotype of the mutants.
Ultimate objectives of the study are to locate the site of amino acid
change in these mutant hemagglutinin molecules which pleiotropically
influence viral virulence, the minor antigenic difference which
distinguishes them, and to determine the mechanism by which viral
replication is so influenced by a single mutational change, and to
investigate the role of this mutation in the epidemiology of swine
influenza virus in its natural host.  It is hoped that these studies will
also aid in defining the exact nature of the receptor site on the
hemagglutinin molecule.

## Key facts

- **NIH application ID:** 3124533
- **Project number:** 5R01AI009304-19
- **Recipient organization:** MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
- **Principal Investigator:** EDWIN D KILBOURNE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $162,513
- **Award type:** 5
- **Project period:** 1975-01-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124533

## Citation

> US National Institutes of Health, RePORTER application 3124533, BIOLOGY AND BIOCHEMISTRY OF INFLUENZA VIRUSES (5R01AI009304-19). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124533. Licensed CC0.

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