# IMMUNOLOGY ACTIVATED ENZYME SYSTEMS OF CELLS

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 1991 · $132,710

## Abstract

The long term objectives of this application are to define in molecular
terms the biochemical and physiological responses of the neutrophil to
chemotactic agents.  In this work, particular stress is laid upon
delineating the nature and role of the formylpeptide chemotactic receptor
of the neutrophil but there is also a broadening of the long term
objectives to include the study of the terminal mechanisms of neutrophil
granule exocytosis.  The initial aim is to extend and refine the model of
the formylpeptide receptor of the rabbit neutrophil to the area of
accommodating a terra, pentapeptides and also longer chain peptides.  In
addition, particular attention will be paid to the nature of the
conformation of the peptide in the receptor using computer simulation to
guide the research.  In this regard also, the basis for the high activity
of the unformulated defivatives of the pentapeptides we have studied will
also be investigated.  The second aim is to study the nature and
mechanism of the release of the specific granule contents in cell free
extracts of rabbit peritoneal neutrophils and attempt to extend these
studies to the release from azurophil granules and to release engendered
by fMet-Leu-Phe-OH stimulation of receptors present in the cell free
neutrophil system. The third aim is to test the hypothesis that prior
incubation with phorbol 12-myristate 13-acetate (PMA) induces inhibition
of neutrophil responses to fMet-Le-phe and other chemotactic factors by
activating protein kinase C to phosphorylate the alpha n subunit of Gn
the G protein that mediates the stimulated receptor activation of
phospholipase C.  The putative phosphorylation causes a shift in the
equilibrium between alpha n and beta gamma to the undissociated
heterotrimer Gn and a loss of Gn function.

## Key facts

- **NIH application ID:** 3124605
- **Project number:** 5R01AI009648-21
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **SAM.gov UEI:** H6D6JMXJXDE6
- **Principal Investigator:** ELMER L BECKER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $132,710
- **Award type:** 5
- **Project period:** 1989-09-30 → 1994-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124605

## Citation

> US National Institutes of Health, RePORTER application 3124605, IMMUNOLOGY ACTIVATED ENZYME SYSTEMS OF CELLS (5R01AI009648-21). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124605. Licensed CC0.

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