# DNA REPLICATION AND RECOMBINATION IN E COLI AND PHAGE

> **NIH NIH R01** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 1987 · $163,923

## Abstract

Two systems of recombination and replication in E. coli and Lambda will be
studied:  RecA dependent RNA replication and Lambda-Red mediated
recombination.  Additional mutants which regulate Rec dependent replication
will be sought and their interactions studied.  The dnaT and sdrT genes
will be cloned and their products isolated.  Using Mud insertions, the
transcription of these genes will be analyzed.  Intermediates in Rec
dependent replication will be studied in vitro, and the role of this
replication system in mutagenesis will be further analyzed.  Using a
"Hyper-Red" recombination prone DNA sequence, inserted into a Lambda
vector, we will study Red mediated deletions and recombination.  This
system will be used to develop an in vitro assay system of Red
recombination, driven by concomitant DNA replication.  This system may be a
prototype for somatic recombination in animal cells and will be used to
assay for the presence of animal cell recombination enzymes.

## Key facts

- **NIH application ID:** 3124621
- **Project number:** 5R01AI010056-18
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **SAM.gov UEI:** LL8GLEVH6MG3
- **Principal Investigator:** KARL Gordon LARK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $163,923
- **Award type:** 5
- **Project period:** 1978-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124621

## Citation

> US National Institutes of Health, RePORTER application 3124621, DNA REPLICATION AND RECOMBINATION IN E COLI AND PHAGE (5R01AI010056-18). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124621. Licensed CC0.

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