# IMMUNOLOGY AND PHYSIOLOGY OF RECIRCULATING LYMPHOCYTES

> **NIH NIH R01** · SUNY DOWNSTATE MEDICAL CENTER · 1988 · $229,400

## Abstract

Lymphocyte recirculation results in the continuous supply of T and B cells
to lymph nodes (LN), intestinal lymphoid tissues (ILT) and spleen and
provides a system for redistribution of lymphocytes within these tissues.
The first step in entry of blood lymphocytes into LN and ILT is the binding
of these cells to venules lined by high endothelium (HEV).  The long term
goal of our research is to elucidate in humans the molecular mechanisms of
lymphocyte adhesion to the high endothelium and to delineate the
relationship of abnormalities in lymphocyte-HEV interactions to disease
processes.  The primary objectives of this proposal are to identify human
high endothelial binding lymphocytes and to analyze surface molecules of
human cells which mediate adhesion.  This function of human lymphocytes
will be assessed through the use of in vitro HEV adherence assays utilyzing
both rat and human HEV.  We will identify the class and subclass of
lymphocytes which bind to these vessels; specific adhesion molecules will
be sought using a rabbit antibody with cross-specie reactivity.  We will
test the hypothesis that different lymphocyte populations and cell surface
structures bind to HEV of LN and HEV of ILT.  We plan to produce mouse
monoclonal antibodies to high endothelial adhesion molecules of human
lymphocytes and will attempt to devise methods for the isolation of viable
high endothelial cells.  The in vitro HEV adherence assay wil be employed
to examine the properties of blood lymphocytes of patients with lymphomas,
leukemia and in immunosuppressed states.

## Key facts

- **NIH application ID:** 3124632
- **Project number:** 5R01AI010080-18
- **Recipient organization:** SUNY DOWNSTATE MEDICAL CENTER
- **SAM.gov UEI:** NJ14V2NZYM68
- **Principal Investigator:** JOSEF MICHL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $229,400
- **Award type:** 5
- **Project period:** 1978-09-01 → 1991-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124632

## Citation

> US National Institutes of Health, RePORTER application 3124632, IMMUNOLOGY AND PHYSIOLOGY OF RECIRCULATING LYMPHOCYTES (5R01AI010080-18). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124632. Licensed CC0.

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