# TRANSFERABLE STREPTOCOCCAL TRANSPOSON, TN916

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1988 · $219,150

## Abstract

The aim of this study is to investigate the molecular basis of the
transposition and fertility of Tn916.  Studies designed to examine
structure and function should reveal details of a transposition process
hypothesized to be significantly different from that of most other well
characterized systems. i.e. transposition of Tn916 may occur by an
excision-insertion event.  Experiments will also test the hypothesis that
Tn916 conjugal transfer actually represents an elaborate transposition
event (where donor and recipient replicons are located in different cells)
and begin to illuminate the mechanisms(s) by which such an event occurs and
is regulated.

More specifically we will:  1) construct a physical map of Tn916; 2)
sequence the ends of Tn916 and those host sequences surrounding the site of
the transposon insertion; 3) determine if Tn916 can transpose in E. coli;
4) isolate transposon excision products which arise in E. coli; 5) examine
behavior of Tn916 in Streptococcus sanguis; 6) examine the production of
Tn916 specific macromolecules; and 7) isolate and map mutants defective in
Tn916 specific functions.

## Key facts

- **NIH application ID:** 3124700
- **Project number:** 5R01AI010318-17
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** DON B CLEWELL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $219,150
- **Award type:** 5
- **Project period:** 1978-09-29 → 1989-09-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124700

## Citation

> US National Institutes of Health, RePORTER application 3124700, TRANSFERABLE STREPTOCOCCAL TRANSPOSON, TN916 (5R01AI010318-17). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124700. Licensed CC0.

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