# GENETICS AND BIOCHEMISRTY OF INTRACELLULAR PROTEOLYSIS

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 1993 · $266,262

## Abstract

The long term goal of this project is to understand in molecular detail
the role of peptidases in cellular physiology.  The specific aims of this
proposal are:  a) To fully characterize the mechanism and physiological
significance of the anaerobic regulation of the S typhimurium pepT gene.
Transcription of this gene is positively regulated by the OxrA(Fnr)
protein and these studies aim to contribute to our understanding of the
mechanism of action of OxrA(Fnr) and of transcriptional activators in
general. b) To elucidate the cellular role of a proteolytic processing
enzyme encoded by the opdA gene.  The product of this gene is required
for a proteolytic processing step in phage P22 and, in mutant form, for
the secretion of certain Escherichia coli signal-sequence-defective
proteins.  c) To develop systems for studying the in vivo degradation of
peptides and unstable proteins.  Such a system will allow the
identification of new peptidase activities and help to define the pathway
by which peptides are degraded in the bacterial cell.

This work aims to contribute to our understanding of the mechanisms of
transcriptional activation and of intracellular protein breakdown.  These
processes are important in all organisms and progress in these areas is
expected to contribute to our understanding of processes vital to human
health.

## Key facts

- **NIH application ID:** 3124705
- **Project number:** 2R01AI010333-23
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **SAM.gov UEI:** Y8CWNJRCNN91
- **Principal Investigator:** CHARLES G MILLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $266,262
- **Award type:** 2
- **Project period:** 1978-04-01 → 1998-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124705

## Citation

> US National Institutes of Health, RePORTER application 3124705, GENETICS AND BIOCHEMISRTY OF INTRACELLULAR PROTEOLYSIS (2R01AI010333-23). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124705. Licensed CC0.

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