# GENETICS AND BIOCHEMISTRY OF INTRACELLULAR PROTEOLYSIS

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 1990 · $95,236

## Abstract

The goal of this project is to understand the pathway by which
intracellular proteins of Salmonella tyohimurium are degraded.  New
knowledge concerning this pathway should contribute to an increased
understanding of the physiological significance of intracellular
proteolysis and should be useful in elucidating similar proteolytic
processes that exist in all types of cells.

Three lines of experimentation will be pursued: 1. A system will
be developed in which the degradation products of a single protein
can be observed.  The gene for the protein to be studied will be
cloned behind a phage T7 promoter so that the protein can be
expressed and radiolabeled in the absence of other protein
synthesis.  The pattern of intermediates in the degradation of this
protein and the effect of mutations in protease genes on this
pattern will be studied.  The specificities of the exopeptidases
already shown to function on the pathway of protein degradation
will be determined using purified enzymes and appropriate peptide
substrates including the intermediates identified in in vivo
experiments.  The results of these studies should define where in
the degradation pathway these exopeptidases function.  2. Genes
coding for endoproteases that may act in the early steps of the
degradation pathway will be identified and inactivated or
overexpressed by mutation.  The effects of these mutations on the
pattern of intermediates observed in 1. will be determined to
define the role of each enzyme in the pathway.  3.  The regulation
of the genes coding for important components of the proteolytic
apparatus will be studied.  The nature of the signals that
determine the levels of expression of these genes may provide
important clues about the physiological significance of
intracellular proteolysis.

## Key facts

- **NIH application ID:** 3124713
- **Project number:** 7R01AI010333-20
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **SAM.gov UEI:** Y8CWNJRCNN91
- **Principal Investigator:** CHARLES G MILLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $95,236
- **Award type:** 7
- **Project period:** 1978-04-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124713

## Citation

> US National Institutes of Health, RePORTER application 3124713, GENETICS AND BIOCHEMISTRY OF INTRACELLULAR PROTEOLYSIS (7R01AI010333-20). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124713. Licensed CC0.

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