# IMMUNOCHEMICAL STUDIES ON GONOCOCCAL SURFACE ANTIGENS

> **NIH NIH R01** · ROCKEFELLER UNIVERSITY · 1987 · $229,647

## Abstract

Gonorrhea remains one of the most common infectious diseases and eludes
control by means other than vaccination.  For a meaningful
immunoprophylactic program, information on the structure-function
relationship of the surface antigens with host components are needed.  The
proposed work consists of determining the primary structure of proteins I,
proteins II, and pili by standard DNA and protein sequencing techniques.
Currently available cloned genes will be used and additional ones will be
isolated by hybridization selection with the cloned genes already
available, with synthetic oligonucleotides, and by immunological selection
of clones expressing the product.  The genetic mechanisms responsible for
intra-strain variability of proteins II will be studied with DNA probes to
variable and constant areas of the molecule.  The folding of protein I will
be studied with bifunctional reagents, and its secondary and tertiary
structure elucidated by spectroscopic and X-ray diffraction techniques.
The steps involved in pilin assembly into fully formed pili will be studied
by chemical and genetic techniques.  The ligand on human cells binding to
gonococcal pili will be defined.

## Key facts

- **NIH application ID:** 3124738
- **Project number:** 5R01AI010615-17
- **Recipient organization:** ROCKEFELLER UNIVERSITY
- **SAM.gov UEI:** LHGDNJMZ64Y1
- **Principal Investigator:** Emil C. Gotschlich
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $229,647
- **Award type:** 5
- **Project period:** 1974-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124738

## Citation

> US National Institutes of Health, RePORTER application 3124738, IMMUNOCHEMICAL STUDIES ON GONOCOCCAL SURFACE ANTIGENS (5R01AI010615-17). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124738. Licensed CC0.

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