# FUNCTIONAL ANTIGENS OF TRICHINELLA SPIRALIS

> **NIH NIH R01** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1987 · $358,000

## Abstract

Two protection-inducing antigens specific to the L1 larva of
Trichinella spiralis will be investigated regarding their structure
and function using immunological, biochemical and molecular
biological approaches.  Expression and genomic libraries in
suitable vectors will be constructed and cloned DNA molecules
encoding both the 48K and 50/55K protein will be isolated.
Expression of positive cDNA clones will be identified by colony
immune assay with polyvalent rabbit anti-48K and 50/55K
antibodies.  Fusion peptides containing epitopes will be tested for
their ability to induce protection in mice.  Southern blot analysis
will determine the genomic arrangement of DNA encoding both
antigens.   Northern analysis on each stage of Trichinella spiralis
(L1-L4 larva, adult and newborn larva) will reveal the stage-
specificity of gene expression for each antigen.  The sequence of
cDNA and genomic DNA for each antigen will be determined.
Both proteins can be phosphorylated, and in addition, the 48K
antigen binds divalent cations, particularly Ca2+.  Thus peptide
mapping and sequencing studies on each molecule will be carried
out to determine the sites of phosphorylation for both proteins,
and the number and sites of Ca2+ binding for the 48K molecule.
Computer analysis of the primary amino acid consensus sequence
and DNA sequence will determine areas of hydrophobicity and
degrees of homology with other known sequences.  Particular
attention to similarities between regulatory proteins and the 48K
and 50/55K antigens will be given, since preliminary data indicate
that they may be involved in regulation of host anerobic
glycolysis.

## Key facts

- **NIH application ID:** 3124743
- **Project number:** 2R01AI010627-17
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** DICKSON DESPOMMIER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $358,000
- **Award type:** 2
- **Project period:** 1977-06-01 → 1991-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124743

## Citation

> US National Institutes of Health, RePORTER application 3124743, FUNCTIONAL ANTIGENS OF TRICHINELLA SPIRALIS (2R01AI010627-17). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124743. Licensed CC0.

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