# GENETICS OF ANTIBODY DIVERSITY, EXPRESSION, & EVOLUTION

> **NIH NIH R01** · CALIFORNIA INSTITUTE OF TECHNOLOGY · 1987 · $245,538

## Abstract

We plan to employ two powerful new methodologies, hybridomas to produce
monoclonal antibodies and newly developed amino acid sequence techniques, to
characterize two multigene families of the mouse, those encoding the antibody
molecules and Ia antigens.  These analyses should provide insights into the
organization, expression and evolution of these two gene families.

We have recently isolated a mouse transplantation antigen by employing a
monoclonal antibody produced by a hybridoma.  Because of a series of
improvements in instrumentation and the chemistry of microsequencing, we have
been able to determine the sequence of the N-terminal 20 residues of the
transplantation antigen without employing radiolabelling techniques.  We hope to
increase our current sensitivity (e.g. approximately 40 residues on 200
picomoles of polypeptide)  by a factor of 10-100-fold within the next year.
These new sequencing and microsequencing techniques will be applied to the
analysis of antibodies and Ia antigens.

## Key facts

- **NIH application ID:** 3124815
- **Project number:** 5R01AI010781-17
- **Recipient organization:** CALIFORNIA INSTITUTE OF TECHNOLOGY
- **SAM.gov UEI:** U2JMKHNS5TG4
- **Principal Investigator:** LEROY E HOOD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $245,538
- **Award type:** 5
- **Project period:** 1974-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124815

## Citation

> US National Institutes of Health, RePORTER application 3124815, GENETICS OF ANTIBODY DIVERSITY, EXPRESSION, & EVOLUTION (5R01AI010781-17). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124815. Licensed CC0.

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