# REGULATORY CIRCUITRY USING DEFINED PROTEIN ANTIGENS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1987 · $179,818

## Abstract

We are studying the interaction of mouse T-helper cells, T-suppressor cells, B
cells, and macrophages in the immune response to two proteins, lysozyme and
beta-galactosidase.  Our overall objective is to ascertain whether there are
differences among these cell types in epitope recognition.  Earlier evidence has
shown us that key suppressive determinants exist on these two proteins.  We will
study how the presentation of antigen by macrophages influences the T-cell
recognition, what specificities are characteristic of T-helper and T-suppressor
subpopulations and how this compares with B cell populations, and how the nature
of the immune response is altered by separating suppressor from helper
determinants, or by fracturing or covering suppressive determinants.  We will
study the effect of anti-idiotypic antibodies raised against B-cell hybridomas
and T cell hybridomas of defined specificity (anti-lysozyme and anti-beta
galactosidase) in shifting the regulatory balance.  We would like to clarify the
crossover between idiotypic and antigenic regulatory universes.  The cellular
target of suppressor cells will be defined.  The major assay system to be
employed are in vitro T-dependent antibody forming systems where our protein
antigens act as carriers for anti-hapten responses, and antigen-dependent
T-proliferation systems using primed lymph nodes and peritoneal-exudate T cells.

## Key facts

- **NIH application ID:** 3124908
- **Project number:** 5R01AI011183-15
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** ELI E SERCARZ
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $179,818
- **Award type:** 5
- **Project period:** 1978-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124908

## Citation

> US National Institutes of Health, RePORTER application 3124908, REGULATORY CIRCUITRY USING DEFINED PROTEIN ANTIGENS (5R01AI011183-15). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124908. Licensed CC0.

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