# REGULATION OF REAGINIC ANTIBODY RESPONSE

> **NIH NIH R01** · LA JOLLA INSTITUTE FOR IMMUNOLOGY · 1992 · $194,240

## Abstract

The purpose of this research is to find some maneuvers to suppress the IgE
antibody response to allergen in an antigen-specific manner.  Previous
studies have shown that a lipomodulin-like lymphokine, i.e., glycosylation
inhibiting factor (GIF), facilitated the generation of antigen-specific
suppressor T cells (Ts), which form their own GIF.  Upon antigenic
stimulation, the antigen-specific Ts form GIF that has affinity for nominal
antigen.  The antigen-binding GIF appears to be identical to antigen-
specific Ts factor (TsF), and composed of antigen-binding chain and
nonspecific GIF.  Based on the findings, we established an immmunological
maneuver to construct Ts hybridomas from antigen-primed murine spleen
cells, and demonstrated that antigen-binding GIF from the hybridomas
suppressed the antibody response of the donor of the spleen cells to
homologous antigen.  As the results of application of the same method to
peripheral blood mononuclear cells of a patient, allergic to bee venom, we
established human T cell hybridomas which produce antigen-binding GIF upon
cross-linking of CD3 on the cells.  Since murine GIF was isolated to
biochemical homogeneity, i) attempts will be made to isolate human
nonspecific GIF, and to biochemically characterize antigen-binding GIF of
both species.  ii) Based on the structure of murine nonspecific GIF, CDNA
clone encoding the lymphokine will be isolated, and the experiments will be
extended to obtain CDNA encoding human GIF.  iii) We shall test the
hypothesis that antigen-binding GIF as well as T cell receptors of the cell
source of the factor recognize a common epitope representing an external
structure in the antigen molecule, and that a certain epitope specificity
of the lymphokine is essential for immunosuppressive effects.  iv)
Finally, mechanisms of immunosuppression by GIF will be investigated in
murine system.  Since our recent studies indicated that GIF suppresses
presentation of antigen by splenic adherent cells to T cells, the effect of
antigen-binding GIF on antigen-presenting cells will be analyzed.

## Key facts

- **NIH application ID:** 3124915
- **Project number:** 2R01AI011202-20A1
- **Recipient organization:** LA JOLLA INSTITUTE FOR IMMUNOLOGY
- **SAM.gov UEI:** ZPAUY3FQMM26
- **Principal Investigator:** KIMISHIGE ISHIZAKA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $194,240
- **Award type:** 2
- **Project period:** 1978-02-01 → 1996-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124915

## Citation

> US National Institutes of Health, RePORTER application 3124915, REGULATION OF REAGINIC ANTIBODY RESPONSE (2R01AI011202-20A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124915. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*