# REGULATION OF REAGINIC ANTIBODY RESPONSE

> **NIH NIH R01** · LA JOLLA INSTITUTE FOR IMMUNOLOGY · 1990 · $249,254

## Abstract

The purpose of this research is to elucidate the mechanisms
involved in the regulation of the IgE response, and to find some
maneuvors to suppress the IgE antibody formation to allergens.
During the current grant period, we found that a lipocortin-like
lymphokine, i.e., glycosylation inhibiting factor (GIF), suppressed
the primary IgE and IgG antibody responses in the mouse, and
diminished the on-going IgE antibody formation.  Analysis of
cellular mechanisms for the immunosuppression indicated that
GIF facilitated the generation of antigen-specific suppressor T
cells which formed their own GIF.  In the present proposal,
attempts will be made to generate antigen-specific suppressor T
cells in vitro from antigen-primed T cells, and to elucidate the
cellular and molecular mechanisms involved.  Experiments will be
carried out to determine whether GIF may change the function of
antigen-primed T cells, and may affect the antigen-presenting
cells.  It was also shown that antigen-specific suppressor T cells
form GIF which has affinity for the antigen, and that the antigen-
specific GIF suppresses the in vivo antibody response in a carrier-
specific manner.  Mouse T cell hybridomas were constructed from
an ovalbumin-primed mouse T cell clone.  One of the T cell
hybridomas form antigen-specific GIF upon incubation with
antigen-pulsed syngenic macrophages.  In this proposal, antigen-
specific GIF will be obtained from the T cell hybridomas, and
physicochemical properties of the molecules will be
characterized.  Comparisons will be made between the antigen-
specific GIF and antigen-specific T suppressor factor (TsF)
described by other investigators.  Analysis will be made to
determine whether the MHC compatibility between the cell
source of antigen-specific GIF and target cells is essential for the
carrier-specific suppression.  Based on the findings obtained in
mouse lymphocyte systems, attempts will be made to generate
antigen-specific suppressor T cells in vitro from the peripheral
blood T cells of allergic patients, and to construct human antigen-
specific T cell hybridomas which will produce allergen-specific
GIF that may suppress the antibody response to the allergen.

## Key facts

- **NIH application ID:** 3124922
- **Project number:** 7R01AI011202-18
- **Recipient organization:** LA JOLLA INSTITUTE FOR IMMUNOLOGY
- **SAM.gov UEI:** ZPAUY3FQMM26
- **Principal Investigator:** KIMISHIGE ISHIZAKA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $249,254
- **Award type:** 7
- **Project period:** 1990-02-01 → 1992-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124922

## Citation

> US National Institutes of Health, RePORTER application 3124922, REGULATION OF REAGINIC ANTIBODY RESPONSE (7R01AI011202-18). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124922. Licensed CC0.

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