# HOST AND VIRAL INFLUENCES ON PHAGE LAMBDA DEVELOPMENT

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1991 · $275,643

## Abstract

The major objective of this proposal is to elucidate how the N protein of
coliphage lambda controls gene expression by modulating transcription
termination.  Components of this transcription proteins called Nus,
transcription termination signals and an antitermination signal, nut.
Genetic studies coupled with physiological experiments are directed toward:

1) characterizing the structure and function of the host NusA and NusE
proteins, 2) identifying a new host factor suspected of being involved in
antitermination, 3) determining if a host protein binds to the boxA
component of the nut RNA signal, and if an antitermination complex formed
at one nut site can be donated in trans to a second nut site, 4) assessing
a new and unexpected finding that the promoter influences nut activity, and
5) extending studies on the nucleic acid signals for transcription
termination.

In a related project, we will extend studies on a host factor, Sip, that
appears to modulate the binding to DNA of the C1 transcription factor of
lambdoid phage P22.

This genetic approach should continue to facilitate biochemical analysis,
and to point the way toward new avenues of study of antitermination.  The
well-characterized lambda N system should continue to serve as a useful
model for studies on eukaryotic expression systems thought to be regulated
by antitermination, such as TAT regulation of HIV expression and c-myc tran
ription.

## Key facts

- **NIH application ID:** 3124944
- **Project number:** 2R01AI011459-19
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** DAVID I FRIEDMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $275,643
- **Award type:** 2
- **Project period:** 1976-09-01 → 1996-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3124944

## Citation

> US National Institutes of Health, RePORTER application 3124944, HOST AND VIRAL INFLUENCES ON PHAGE LAMBDA DEVELOPMENT (2R01AI011459-19). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3124944. Licensed CC0.

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