# HUMAN T CELL SUBSETS--ISOLATION AND CHARACTERIZATION

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1988 · $293,355

## Abstract

The overall aims of the present proposal are directed at the isolation and
characterization of unique and still poorly characterized subpopulations of
human T lymphocytes with the view that such cells may be centrally involved
in the circuits which both up and down regulate the human immune response.
The intention is to take advantage of major recent developments in methods
for the generation of antibodies to cell surface antigens, for the
isolation of purified subpopulations of cells, for the cloning of distinct
populations of cells, and lastly for newer methods for the functional
analysis of these populations of cells.  In the present studies, we plan to
undertake a detailed analysis of the phenotypic and functional properties
of unique subpopulations of human T lymphocytes.  In particular, we plan to
develop monoclonal antibody probes to cell surface molecules which can
divide the human inducer and suppressor populations into distinct subsets.
To accomplish this, we plan to develop immunization and screening
strategies which utilize primate lymphocyte populations, subsets of human
peripheral T cells and cloned populations of functionally unique
populations of human T cell lines.  Once these antibodies are identified,
we will separate both the inducer and suppressor populations into
phenotypically unique subsets and investigate each for their regulatory
effects on other human T, B and myeloid cells.  The antibodies defined
above will be utilized to characterize biochemically the cell surface
structures they define.  Moreover, attempts will be made to elucidate the
function of the structures characterized.  These antibodies will be useful
in dissecting and characterizing patients with autoimmune and
immunodeficiency diseases where the immunoregulatory circuit is clearly
abnormal.  Lastly, we will identify minor populations of T lymphocytes
present both in normal peripheral blood and which can be cultured and
determine whether these minor populations have distinct and unique
functional programs.

## Key facts

- **NIH application ID:** 3125103
- **Project number:** 5R01AI012069-15
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** STUART F SCHLOSSMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $293,355
- **Award type:** 5
- **Project period:** 1978-10-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125103

## Citation

> US National Institutes of Health, RePORTER application 3125103, HUMAN T CELL SUBSETS--ISOLATION AND CHARACTERIZATION (5R01AI012069-15). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125103. Licensed CC0.

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