# NEW MICROBIAL VITAMIN - COENZYME RELATIONSHIPS

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 1988 · $101,244

## Abstract

The purpose of the propsed research is to elucidate the biochemistry,
physiology, and structure of new coenzymes and vitamins that have been
discovered in methanogenic bacteria.  In the history of biochemistry,
microbbes have played a pivitol role in the assay of viatamins as well as
in the elucidation of the biochemical function of vitamins and coenzymes.
To completely understand cell chemistry is important, for biochemical
deficiencies produce a diseased state.  Study of methanogens have revealed
7 new factor-coenzyme relationships.  Study of these coenzymes is providing
new insights into biochemical reactions.  To study these coenzymes
methanogens are mass cultered in kilogram quantities on H2 + CO2.
Coenzymes are frationated from cell extracts to homogeniety, and their
structure is then determined by use of mass spectrometry, 1H-NMR, 13C-NMR,
elemental analysis and other analytical procedures.  The biochemical role
of each coenzyme in enzymatic catalysis is studied theough purification of
the enzymes that requires the coenzyme for catalytic activity.  Methanogens
are only distantly related to typical bacteria, and because of their
unusual metabolism are providing unique handles by processing certain
coenzymes in unusually high concentrations.

## Key facts

- **NIH application ID:** 3125150
- **Project number:** 5R01AI012277-15
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **SAM.gov UEI:** Y8CWNJRCNN91
- **Principal Investigator:** RALPH S WOLFE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $101,244
- **Award type:** 5
- **Project period:** 1974-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125150

## Citation

> US National Institutes of Health, RePORTER application 3125150, NEW MICROBIAL VITAMIN - COENZYME RELATIONSHIPS (5R01AI012277-15). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125150. Licensed CC0.

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