# MOLECULAR BIOLOGY OF NDV MEMBRANE PROTEINS

> **NIH NIH R01** · UNIV OF MASSACHUSETTS MED SCH WORCESTER · 1987 · $174,993

## Abstract

Newcastle disease virus (NDV) is the model for important, but difficult to
study paramyxoviruses like measles, mumps, and respiratory syncytial
viruses.  It also remains an avian pathogen which produces frequent
devastating epizootics in poultry flocks throughout the world.  In this
proposal we utilize mutants and revertants and monoclonal antibodies to
explore NDV's three membrane proteins (HN, F and M); particular emphasis
will be placed on HN.  These proteins have multiple functions, some of
which have been clearly defined and others of which have not.  We will
attempt to assign protein(s) to functions where this hasn't been done and
locate functional areas as well as monoclonal antibody binding sites on the
molecules.  Using wild type NDV, AV-WT, we will fragment HN, identify
peptides and sites of disulfide bonds or glycosylation, and sequence
important peptides.  Comparing AV-WT and mutant proteins we will map
mutations.  We will use monoclonal antibodies to these proteins in
functional inhibition and peptide binding studies to locate functional
areas.  We will also use monoclonal antibodies to probe changes in mutant
protein binding sites, and in the case of anti-HN antibodies, to select
site-specific mutants with alterations in regions important to
infectivity.  As mutants and antibodies to F become available, we will
conduct similar studies on F.  Finally, we will conduct additional studies
on mutants and revertants to confirm the assignment of group D mutants to
the M protein.  We will probe previously undetected processing of the HN
protein and examine HN's role(s) in hemolysis and infectivity.

## Key facts

- **NIH application ID:** 3125197
- **Project number:** 5R01AI012467-11
- **Recipient organization:** UNIV OF MASSACHUSETTS MED SCH WORCESTER
- **SAM.gov UEI:** MQE2JHHJW9Q8
- **Principal Investigator:** MICHAEL A. BRATT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $174,993
- **Award type:** 5
- **Project period:** 1978-09-01 → 1988-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125197

## Citation

> US National Institutes of Health, RePORTER application 3125197, MOLECULAR BIOLOGY OF NDV MEMBRANE PROTEINS (5R01AI012467-11). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3125197. Licensed CC0.

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