# ANALYSIS OF HUMAN CYTOMEGALOVIRUSES

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1987 · $120,348

## Abstract

The overall objectives of this project are to designate human
cytomegalovirus (CMV)-specific gene functions and to express DMV-specific
antigens in prokaryotic and eukaryotic systems for future development of
diagnostic antigens and the CMV subunit viral vaccine in order to prevent
and control cytomegalovirus infection.  The long term objectives will be
achieved through the following specific approaches.  (1)  To continue to
construct the detailed restriction enzymes cleavage map of L repeat, S
repeat and L-S junction fragments of DMV genome, and to search for and
characterize the possible putative "a" and "c" sequence in CMV genomes by
nucleic acid hybridization and DNA sequencing techniques.  The possible
origin of DNA replication in putative "c" sequence will be examined in
yeast system and in human fibroblast for its autonomous replication
sequence.  (2)  To map genes coded for the virus-induced DNA polymerase,
ribonucleoside reductase, virus-specific structural polypeptides (including
94K, 150K and other glycopolypeptides, etc.) using various expressive
vectors of E. coli (pDR540, pDR720, pVE-J001 and Lambdagt11 and of
mammalian cell (e.g. pSVOH in COS-1 cell), and various available monoclonal
antibodies generated in our laboratory.  (3)  To complete the
classification and characerization of monoclonal antibodies generated
against purified virus and infected cell lysate for gene cloning and the
development of diagnostic reagent.  (4)  To express CMV specific
non-glycosylated polypeptides in E. coli using expression vectors which
carry strong tac and trp promoters (e.g. pDR540, pDR720 and pVEJ1001), and
glycosylated (also non-glycosylated) polypeptides in yeast using expression
vectors carrying a strong alcohol dehydrogenase promoter (pAAH-5 and
pMA56).  Finally, (5)  the viral polypeptides which are responsible for the
induction of a major humoral immune response will be determined by Western
blotting, immunoprecipittion, and SDS-PAGE.  The antigenic determinants
will be studied further by proteolytic digestion and Western blot
immunoreaction.  The DNA fragments encoding these polypeptides will be
engineered and cloned for studying the feasibility of developing the
subunit viral vaccine.

## Key facts

- **NIH application ID:** 3125266
- **Project number:** 5R01AI012717-11
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** Eng-Shang Huang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $120,348
- **Award type:** 5
- **Project period:** 1978-06-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125266

## Citation

> US National Institutes of Health, RePORTER application 3125266, ANALYSIS OF HUMAN CYTOMEGALOVIRUSES (5R01AI012717-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125266. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*