# INTERFERENCE BY DEFECTIVE INFLUENZA VIRUSES MECHANISM

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1988 · $156,591

## Abstract

Our long term goal is to elucidate the structure and genesis of defective
interfering (DI) influenza virus, the mechanism of DI influenza virus
mediated interference and hopefully, the role of the DI virus in the
evolution and the diversity of influenza viruses in nature.  In this
present project our specific objectives are to further investigate the
structure of DI RNA and its relationship to progenitor RNAs, to investigate
the transcription and translation products of DI RNA in vitro and in
infected cells, to establish an in vitro system of influenza viral RNA
replication, and finally to determine the role of DI viruses viral
persistency and diversity.

DI virus from influenza A, B and C type viruses will be produced by passing
virus at a high multiplicity using productive cell-virus systems.  DI
viruses will be amplifed using homologous standard virus as helper.
Detailed structure analyses of DI RNA and their relationship to the
progenitor genes will be performed by DNA cloning, heteroduplex mapping,
restriction analyses and sequence determination.

The mechanism of DI virus mediated interference will be elucidated by
studying the transcription and replication of DI viral RNA in vitro as well
as in infected cell.  Subsequently, the effect of DI virus on the
transcription and replication of standard viral RNAs will be determined
both in vitro replication and transcription systems as well as in cells
coinfected with both DI virus and standard virus.

The role of DI viruses will be investigated by isolating variant viruses
resistant DI mediated interference and determining the gene(s) responsible
for the resistance.

## Key facts

- **NIH application ID:** 3125279
- **Project number:** 5R01AI012749-13
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** DEBI P. NAYAK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $156,591
- **Award type:** 5
- **Project period:** 1975-06-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125279

## Citation

> US National Institutes of Health, RePORTER application 3125279, INTERFERENCE BY DEFECTIVE INFLUENZA VIRUSES MECHANISM (5R01AI012749-13). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125279. Licensed CC0.

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