# CELL INTERACTIONS IN IMMUNE RESPONSES IN VITRO

> **NIH NIH R01** · JEWISH HOSPITAL OF SAINT LOUIS · 1987 · $199,633

## Abstract

The overall goals of this project are to characterize cells of the murine
system and determine the mechanism(s) of action and interactions among
these cells in antibody responses to an antigen under immune response (Ir)
gene control-L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT).  T cells are
critical for the development and regulation of immune responses; the
understanding of the diversity and biological activity of T cell subsets is
extensive, but little is known about T cell receptors for antigen or how
regulatory T cells act.  The objectives of this proposal are to identify
and characterize regulatory T cell subsets in responder and nonresponder
mice and (responder X nonresponder) F1 and (responder X responder)F1 mice
after immunization with soluble GAT and GAT-macrophages.  We have
identified genetically restricted Lyt 1+, I-J- helper T cells, Lyt 1+, I-J+
suppressor-inducer and Lyt 2+, I-J+ suppressor effector T cells in
(responder X nonresponder)F1 immunized with soluble GAT.  Similar
regulatory T cell subsets are in (responder X responder)F1 mice and are
expected in parental responder and nonresponder mice.  Our strategy is to
establish cloned T cell lines representative of these regulatory T cell
subsets and to characterize these clones by membrane antigen phenotype (Lyt
1, 2, 3; I-A; I-J; Qa 1; etc.), function and antigen-binding capacity.  We
plan to identify, isolate and characterize molecules in the membranes of
these T cell clones which serve as recognition units or receptors for
antigen and Ia restriction elements.  New monoclonal serological reagents
will be developed for this analysis by immunization with T cell clones.  In
addition, the mechanism(s) of action and interaction of the regulatory T
cell clones will be determined to probe the cellular site and mechanism of
expression of Ir gene function in antibody responses to GAT.  These studies
should lead to the identification of all essential T cell subsets involved
in the regulation of antibody responses to one antigen under Ir gene
control and provide insight into the nature of T cell receptors and the
mechanism of Ir gene regulation of immunity.

## Key facts

- **NIH application ID:** 3125568
- **Project number:** 5R01AI013915-12
- **Recipient organization:** JEWISH HOSPITAL OF SAINT LOUIS
- **Principal Investigator:** CARL W PIERCE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $199,633
- **Award type:** 5
- **Project period:** 1978-09-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125568

## Citation

> US National Institutes of Health, RePORTER application 3125568, CELL INTERACTIONS IN IMMUNE RESPONSES IN VITRO (5R01AI013915-12). Retrieved via MORVS 2026-10-26 from https://api.morvs.ai/grant/nih/3125568. Licensed CC0.

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