# MITOCHONDRIAL DNA OF NORMAL AND MUTANT TRYPANOSOMES

> **NIH NIH R01** · SEATTLE BIOMEDICAL RESEARCH INSTITUTE · 1989 · $286,815

## Abstract

The overall objective is to elucidate the mechanisms which regulate the
expression of mitochondrial and nuclear genes during the life cycle of
African trypanosomes and explore the mechanisms which coordinate these
genetic regulatory processes.  The immediate objectives are to characterize
the expression of mitochondrial (maxicircle) and nuclear genes at the
levels of transcription, transcript processing, and translation.  The
expression of mitochondrial and nuclear genes will be examined at these
levels in different life cycle stages and stocks of Trypanosoma brucei to
discern the mechanisms regulating these cell differentiations.  Experiments
are proposed to:

1. Characterize maxicircle transcripts that are diferentially expressed
during the life cycle and those which differ among stocks.  These
experiments are designed to elucidate the mechanisms that regulate the
differential expression of maxicircle genes that was discovered during the
previous grant period and the mechanisms of maxicircle transcription and
transcript processing.

2. Identify proteins encoded by the maxicircle and characterize their
production in different life cycle stages.  These experiments are designed
to establish the relationship between the presence of specific maxicircle
transcripts and mitochondrial proteins and hence mitochondrial function.

3. Identify and characterize nuclear genes that are differentially
expressed during the life-cycle.  Gene encoding proteins with varying
physical and functional associations with proteins encoded by the
maxicircle will be studied.

4. Characterize the expression of nuclear genes that are differentially
expressed during the life cycle at the genomic level and at the levels of
transcription, transcript processing and translation.  These studies will
be done in parallel with analogous studies of maxicircle genes in order to
elucidate the genetic regulatory mechanisms that coordinate the control of
these two genetic systems.

## Key facts

- **NIH application ID:** 3125640
- **Project number:** 5R01AI014102-14
- **Recipient organization:** SEATTLE BIOMEDICAL RESEARCH INSTITUTE
- **Principal Investigator:** KENNETH D STUART
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $286,815
- **Award type:** 5
- **Project period:** 1978-09-01 → 1991-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125640

## Citation

> US National Institutes of Health, RePORTER application 3125640, MITOCHONDRIAL DNA OF NORMAL AND MUTANT TRYPANOSOMES (5R01AI014102-14). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125640. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*