# INVESTIGATIONS OF HUMAN COMPLEMENT

> **NIH NIH R01** · IMMUNE DISEASE INSTITUTE, INC. · 1988 · $227,759

## Abstract

The overall objective of the planned research is the further definition of
genetic control of proteins in the human complement system.  In particular,
subtypes of common C4A and C4B alleles will be sought in order to provide
more informative complotypes for the identification of extended major
histocompatibility complex haplotypes as markers for disease
susceptibility, for immune response and immune suppression genes and for
previously isolated human populations.  The subtypes of common C4A and C4B
alleles will be identified by isoelectric focusing of desialated whole
molecules and by apparent size polymorphisms in C4 Beta and Gamma chains
detected by sodium dodecyl sulfate electrophoresis.  Any such variation
detected will be studied in families to provide allele frequencies,
evidence for inheritance and relationship to other major histocompatibility
complex markers, including restriction fragment length polymorphisms in
genomic DNA.  Other studies will define genetic polymorphism in human Clq
and Cls.  For Clq, charge variation in A, B and C subunits will be
investigated and localized to collagen-like stalk and stem or to globular
head fragments.  For Cls, the variation will be localized to one chain or
the other of Cls, inheritance will be shown in family studies and
population allele frequencies will be determined.  Linkage relationships
will be studied among possible loci for the subunits of Cq, between these
and that for Cls, and between the Cl loci and other complement loci.  From
these studies, we hope to obtain new insights into genetic control of C4,
Clq and Cls and into the evolution of the complement system in man.

## Key facts

- **NIH application ID:** 3125657
- **Project number:** 5R01AI014157-17
- **Recipient organization:** IMMUNE DISEASE INSTITUTE, INC.
- **Principal Investigator:** CHESTER Allan ALPER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $227,759
- **Award type:** 5
- **Project period:** 1977-12-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125657

## Citation

> US National Institutes of Health, RePORTER application 3125657, INVESTIGATIONS OF HUMAN COMPLEMENT (5R01AI014157-17). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125657. Licensed CC0.

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