# CELL MEDIATED IMMUNITY IN INFLUENZA

> **NIH NIH R01** · WISTAR INSTITUTE · 1991 · $203,600

## Abstract

Studies continue to focus on the role of cytotoxic T lymphocytes (CTL) in
host immunity to viruses, using the murine response to type A influenza
viruses as a model system.  In previous work we have shown that recombinant
vaccinia viruses containing cloned influenza virus genes can be used to
both stimulate cytotoxic T lymphocytes (CTL) in vivo and in vitro, and to
sensitize target cells for lysis by anti-influenza virus CTL.  We found
that contrary to expectations, a viral internal protein is recognized by
anti-influenza CTL more efficiently than the major viral glycoprotein.
Experiments will be continued at both population and clonal levels using
vaccinia recombinants containing 7 influenza virus structural genes and 3
non-structural genes to further define the importance of individual virus
gene products in the anti-influenza virus CTL response.  We also propose to
further study the role of CTL in modulating lethal and sublethal influenza
pneumonia.  The in vivo activities of CTL populations and cloned CTL cell
lines will be correlated with their specificity for individual viral
components and their production of gamma interferon.  These studies will
include an investigation of the immune mechanisms underlying our finding
that immunization with recombinant vaccinia viruses protects mice against
lethal influenza pneumonia.  Finally, we propose to study the influence of
the major histocompatibility complex in the recognition of individual viral
components.  This will include determining the class I MHC restruction
elements for each of the viral components recognized as well as an
examination of the phenomenon of MHC associated CTL low responsiveness to
influenza virus.

## Key facts

- **NIH application ID:** 3125667
- **Project number:** 5R01AI014162-14
- **Recipient organization:** WISTAR INSTITUTE
- **SAM.gov UEI:** DW1XZMGNFBL4
- **Principal Investigator:** CHARLES HACKETT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $203,600
- **Award type:** 5
- **Project period:** 1977-08-01 → 1991-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125667

## Citation

> US National Institutes of Health, RePORTER application 3125667, CELL MEDIATED IMMUNITY IN INFLUENZA (5R01AI014162-14). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125667. Licensed CC0.

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