# GENETICS OF HOST FACTORS IN INTEGRATIVE RECOMBINATION

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1988 · $150,024

## Abstract

The objective of this study is to characterize the biological activities of
certain bacterial-encoded DNA binding proyeins.  These proyeins include
IHF, ahe product of yhe himA and hip genes, and the B subunit of DNA
gyrase, encoded by the gyrB gene, which were originally identified by their
roles in the site-specific recombination reaction leading to integration of
the coliphage lambda genome into the E. coli chromosome.

A number of studies directed towards this goal are outlined:  1)  By
employing mutants defective in one or more of these genes, the roles of the
proteins in site-specific recombination, gene expression, transposition of
genetic elements and plasmid maintenance will be studied.  2)  Using both
in vivo and in vitro recombination techniques, hybrid himA-hip genes will
be generated and the biological activity of the resulting chimeric proteins
will be studied in order to relate structure to function of the IHF
subunits.  3)  The roles of IHF and a newly identified alternative host
factor in cleavage and packaging of phage DNA will be explored by analyzing
lambda mutants that overcome blocks in these reactions imposed by himA-hip
and gyrB mutations as well as site mutations on the Lambda genome.  4)
Additional factors required for IHF and/or Gyr activity will be searched
for by selecting mutations that suppress the effect of himA-hip and gyrB
mutations on gene expression.  5)  Possible himA and hip genes from other
bacterial species will be identifed by DNA hybridization and molecular
cloning.

Use of this model system should provide general information useful to the
analysis of viral integration and gene expression in malignant
transformation.  These studies should also yield information on the factors
involved in the transposition of genetic elements such as those encoding
antibiotic resistance markers.

## Key facts

- **NIH application ID:** 3125709
- **Project number:** 5R01AI014363-12
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** DAVID I FRIEDMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $150,024
- **Award type:** 5
- **Project period:** 1977-08-01 → 1990-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125709

## Citation

> US National Institutes of Health, RePORTER application 3125709, GENETICS OF HOST FACTORS IN INTEGRATIVE RECOMBINATION (5R01AI014363-12). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125709. Licensed CC0.

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