# RNA METABOLISM IN HERPES SIMPLEX VIRUS INFECTED CELLS

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1988 · $88,194

## Abstract

There are currently 4 major areas of research in the laboratory, all involving
herpes simplex virus.
The first area is concerned with the structure of replicating viral DNA.  Our
goal is to understand more fully the mechanism whereby the large genome (10 to
the 8th power mol.wt.) is replicated.  These studies are being pursued in
infected cells as well as in an in vitro system, developed in the lab.  The
second area concerns the modification of viral DNA prior to its involvement in
transcription and replication, and the processing and encapsidation of progeny
viral DNA.  These studies are utilizing micrococcal nuclease sensitivity of
viral DNA as a measure of the association of viral DNA with virus specified
structural (virion) and non-structural proteins.  The third area is concerned
with the mechanism whereby the virus shuts off host protein synthesis and
regulates its own temporal program of protein synthesis.  Analyses of polysome
associated mRNA populations in reticulocyte lysates is the major analytical tool
in these studies.  The fourth involves the study of viral specific
transcription.  We are taking two approaches in this regard:  the first is to
develop or adapt nuclear monolayer or totally cell free in vitro systems for
analyzing the requirements for transcription initiation at specific (presumably
correct) promoter sites.  The second approach is to clone small fragments of
viral DNA into plasmid vectors, screen them for transcription initiation sites
in the cell-free system mentioned above and begin comparative nucleotide
sequencing studies within and between groups of coordinately controlled
promoters.

## Key facts

- **NIH application ID:** 3125724
- **Project number:** 5R01AI014377-09
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** STEVEN L BACHENHEIMER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $88,194
- **Award type:** 5
- **Project period:** 1978-07-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125724

## Citation

> US National Institutes of Health, RePORTER application 3125724, RNA METABOLISM IN HERPES SIMPLEX VIRUS INFECTED CELLS (5R01AI014377-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125724. Licensed CC0.

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