# INFLUENCE OF H-2 ANTIGENS ON T CELL RESPONSES

> **NIH NIH R01** · YALE UNIVERSITY · 1989 · $39,678

## Abstract

The purpose of the present studies is to identify cells bearing Ia antigens
encoded in the I region of the murine major histocompatibility complex
(MHC) which influence the development and function of self Ia recognizing
Lyt-1+ helper T cells.  We anticipate studying four such cells:  Veto
cells, which are involved in clonal deletion of autoreactive T cells;
Thymic epithelial cells, which are involved in expansion of self Ia
recognizing Ly1 T cell precursors; Antigen presenting cells, which are
involved in antigen-specific expansion of self Ia recognizing T cells in
the periphery; and B cells, which are activated to proliferate and to
secrete antibody in the presence of Ly1 helper T cells.  We will identify
and determine the functional activity of these cells with cloned T cell
lines of known self and non-self Ia recognition capabilities, with
monoclonal anti-Ia antibodies, and with monoclonal antibodies to other
differentiation antigens, such as surface Ig on B cells.  We will also test
the interactions of each Ia bearing cell type with pre-T cells, with
thymocytes, and with mature peripheral T cells.  The importance of Ia
antigen density on the surface of each cell type will be analyzed using F1
hybrid mice that express Ia antigen alleles unequally, allowing us to
manipulate the density of Ia on cell surfaces in a defined fashion.  We
will examine the induction of tolerance in mature Ly1 T cells and
suppressor T cells activated by interaction with such cloned helper T cell
lines.

From this study, a clear understanding of self tolerance and of the
selective activation of self Ia recognizing helper T cells should emerge.
This, in turn, should clarify the mechanism of action of Immune response
genes (Ir genes), which in many instances appear to reflect tolerance to
self antigens in the context of self Ia glycoproteins.  Information about
Ia antigen functions in mice should give insight into the influence of HLA
antigens on disease susceptibility in man.

## Key facts

- **NIH application ID:** 3125796
- **Project number:** 3R01AI014579-11S1
- **Recipient organization:** YALE UNIVERSITY
- **SAM.gov UEI:** FL6GV84CKN57
- **Principal Investigator:** CHARLES A JANEWAY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $39,678
- **Award type:** 3
- **Project period:** 1978-02-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125796

## Citation

> US National Institutes of Health, RePORTER application 3125796, INFLUENCE OF H-2 ANTIGENS ON T CELL RESPONSES (3R01AI014579-11S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125796. Licensed CC0.

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