# REPLICATION AND INTERFERENCE OF RNA VIRUSES

> **NIH NIH R01** · UNIVERSITY OF FLORIDA · 1988 · $201,357

## Abstract

We plan a systematic, detailed comparative study of the mechanism of
replication of the RNA genomes of two different negative strand viruses,
vesicular stomatitis virus (VSV), a rhabdovirus and Sendai virus, a
paramyxovirus.  We will carry out these studies with the use of an in vitro
reconstitution assay for RNA genome replication that we have recently
developed, utilizing nucleocapsids derived from either the defective
interfering particles of each virus or the wild type viruses as templates.
We shall determine if the requirements for initiation are the same as
those  for elongation during RNA replication.  For each virus we shall
identify and purify the individual viral proteins required for the various
steps of RNA replication and encapsidation.  We also propose to develop an
assay by which we can identify and purify any host cell factors which may
be involved specifically in the replication of the RNA genomes of these
viruses.  We plan to select hybridomas secreting monoclonal antibodies to
the VSV and Sendai nucleocapsid proteins, and also to isolate and use amber
mutants in these proteins, as approaches to understanding the specific
functions of the individual viral nucleocapsid proteins in RNA replication.

## Key facts

- **NIH application ID:** 3125809
- **Project number:** 5R01AI014594-12
- **Recipient organization:** UNIVERSITY OF FLORIDA
- **SAM.gov UEI:** NNFQH1JAPEP3
- **Principal Investigator:** Sue A. Moyer
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $201,357
- **Award type:** 5
- **Project period:** 1978-06-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125809

## Citation

> US National Institutes of Health, RePORTER application 3125809, REPLICATION AND INTERFERENCE OF RNA VIRUSES (5R01AI014594-12). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125809. Licensed CC0.

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