# SYNTHESIS IN NORMAL, VIRUS INFECTED AND TUMOR CELLS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 1987 · $266,545

## Abstract

Overall we will continue studes of persistent infection and of the evolution of
standard RNA virus and of D1 particles during long term persistence.

We will continue to derive molecular clones of standard VSV, D1 particles and
mutated virus from persistent infection.  These will be used to help
characterize and to sequence the biologically interesting mutants recovered from
persistently infected cells.  We will continue to isolate viral nucleocapsids
from persistently infected cells and analyze these to gain insights into events
occuring within persistently infected cells of all kinds.  We will particularly
be interested in ratios of viral to D1 nucleocapside, rates of synthesis,
evidence for cyclic synthesis etc. In various types of persistence.  We will
continue our studies of persistently infected cells in vivo in nude mice, with
specific emphasis upon the role of NK cells and interferon in rejection.  We
will continue our collaboraion with Drs. Reid and Bloom and colleagues at Albert
Einstein Medical Center for these studies.  We will determine the extent to
which standard virus in persistent VSV infections is able to continue mutating
to escape interference by the newly generated D1 particles arising after many
years of persistence.  The mechanisms of this escape from D1 particle
interference will be explored by cross-protection studies matching various D1
particles and virus mutants.

## Key facts

- **NIH application ID:** 3125818
- **Project number:** 5R01AI014627-20
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **SAM.gov UEI:** UYTTZT6G9DT1
- **Principal Investigator:** JOHN J HOLLAND
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $266,545
- **Award type:** 5
- **Project period:** 1978-05-01 → 1988-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125818

## Citation

> US National Institutes of Health, RePORTER application 3125818, SYNTHESIS IN NORMAL, VIRUS INFECTED AND TUMOR CELLS (5R01AI014627-20). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125818. Licensed CC0.

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