# B LYMPHOCYTES: DIFFERENTIATION AND TRIGGERING

> **NIH NIH R01** · TUFTS UNIVERSITY BOSTON · 1988 · $234,121

## Abstract

The aim of the proposed studies is to analyze the role of the murine Fc
receptor complex in B cell differentiation and triggering.  Specifically,
we will attempt to clone and sequence the gene coding for Lym20.2, a
serologically detectable B cell marker that represents an allelic form of
the Fc receptor molecule.  This gene is either identical or very closely
linked to the gene coding for the Mls molecule that has important T cell
triggering functions.

The precise analysis of the genomic organization and function of this
cluster of B cell differentiation antigens will give insights into the
molecular mechanism of T cell-B cell interaction.  Furthermore, it will
provide gene probes that allow the characterization of human B cell
malignancies, which represents the long term significance of this project.

We will construct genomic transfection libraries in heterologous species of
fibroblasts, using chromosomal DNA from Lym20.2+ tissue, and select for
stable transfectants that express Lym20.  We will then prepare specific
subtractive cDNA probes, in order to screen cDNA and genomic cosmid
libraries for the Lym20 gene.  In parallel, we will use synthetic
oligonucleotide probes whose sequence is deduced from a partial amino acid
sequence of the Lym20.2 molecule.

Having established the identity of the selected cDNA and cosmid clones in
chromosomal mapping and transfection experiments, we will analyze the
relationship between the Lym20/Fc receptor molecule and the Mls gene
product.

Finally, we will analyze the mechanism by which Mls alleles control
induction of T cell proliferation.

## Key facts

- **NIH application ID:** 3125925
- **Project number:** 5R01AI014910-11
- **Recipient organization:** TUFTS UNIVERSITY BOSTON
- **SAM.gov UEI:** C1F5LNUF7W86
- **Principal Investigator:** Brigitte T. Huber
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $234,121
- **Award type:** 5
- **Project period:** 1978-04-01 → 1989-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125925

## Citation

> US National Institutes of Health, RePORTER application 3125925, B LYMPHOCYTES: DIFFERENTIATION AND TRIGGERING (5R01AI014910-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125925. Licensed CC0.

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