# DIFFERENTIATION ANTIGENS ON HUMAN T CELL SUBCLASSES

> **NIH NIH R01** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1988 · $242,661

## Abstract

During the last several years this laboratory has continued to be
intimately involved in the development of human lymphocyte cell separation
procedures, human lymphocyte functional assays, T cell cloning strategies,
T cell hybridization and the assessment of T cell surface molecules using
monoclonal antibodies that together permit precise analysis of human immune
responses in vitro.  More recently, our laboratory has begun to utilize
DNA-mediated transfer of genes encoding human T cell surface molecules to
further explore functional properties of human T cells.  The thrust of the
current proposal is to bring to bear these newer technologies to analyze
the relationship of the function of T cell surface molecules to the
cellular interactions important in antigen recognition, T cell triggering
and immunoregulation.  Recent data from our laboratory and others strongly
suggest that many if not all of the already defined surface molecules on
human T cells, including the T1, T3, T4, T8 and T11 molecules themselves,
as well as recently defined activation antigens, serve as critical
structures important in lymphocyte function.  Our specific aims are to:
(1) further define the heterogeneity of cellular functions contained within
the mutually exclusive T4+ and T8+ subsets of mature human T cells.  In
these studies we will also continue to analyze the T-T, T-B, and
T-macrophage cellular imteractions important in the functions of isolated
subsets; (2) further define the precise functional role of T4+ and T8+
glycoprotein molecules themselves in antigen recognition and MHC
restriction as well as helper and suppressor functions; and (3) further
define the functional role of T cell glycoproteins expressed on the
majority of human T cells including T1, T3 and T11 antigens.  This will
involve functional studies to determine the importance of these molecules
in T cell triggering, activation and recognition.  In many of these studies
we should emphasize that we plan to analyze not only freshly isolated
populations of human T cells but also IL-2 dependent T cell clones and
murine fibroblasts expressing human T cell glycoproteins after DNA mediated
transfer of genes encoding these molecules.  These studies will be
essential to further the understanding of human diseases involving
immunodeficiency and/or imbalances in the regulation of T cell function
including AIDS, autoimmune states and neoplastic states.

## Key facts

- **NIH application ID:** 3125951
- **Project number:** 5R01AI014969-11
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** Leonard Chess
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $242,661
- **Award type:** 5
- **Project period:** 1978-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125951

## Citation

> US National Institutes of Health, RePORTER application 3125951, DIFFERENTIATION ANTIGENS ON HUMAN T CELL SUBCLASSES (5R01AI014969-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125951. Licensed CC0.

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