# IMMUNITY MECHANISMS IN HERPESVIRUS INFECTIONS

> **NIH NIH R01** · UNIVERSITY OF TENNESSEE KNOXVILLE · 1988 · $187,652

## Abstract

The goals of our research are to further define immunity mechanisms,
particularly T cell mediated immunity against herpes simplex virus (HSV)
and how best to stimulate protective immunity with purified glycoprotein
(gp) antigens.  Mice will be immunized with HSV and at various times post
infection their spleens or lymph node cells will be taken and stimulated in
vitro with various antigen preparations to generate cytotoxic T lymphocytes
(CTL) and to enumerate CTL precursors and helper T cell precursors by
Poisson statistical analysis of limiting dilution assays.  Liposomes
containing defined HSV gp and appropriate H-2 antigens will be used to
investigate the role of different gp at inducing CTL and other T cell
responses in vitro.  Glycoprotein containing liposomes will also be used to
introduce gp into target cells to ascertain which gp act as target antigens
for CTL.  Experiments are planned in vivo to test the efficacy of
glycoprotein subunit vaccines incorporated into liposomes along with
appropriate adjuvants (such as muramyl dipeptide) at inducing CTL
responses.  The role of CTL of different HSV antigen specificities, T cell
hybridomas and helper T cells in recovery from primary and recrudescent
infection in model systems will also be investigated.

Finally in vitro approaches will be used to determine how anti HSV CTL
responses are regulated, in particular the role of suppressor cells and
factors in the induction process.

The results of our research should help in the development and evaluation
of DNA free subunit glycoprotein vaccines for use against different
diseases caused by HSV.

## Key facts

- **NIH application ID:** 3125960
- **Project number:** 5R01AI014981-11
- **Recipient organization:** UNIVERSITY OF TENNESSEE KNOXVILLE
- **SAM.gov UEI:** FN2YCS2YAUW3
- **Principal Investigator:** Barry T. Rouse
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $187,652
- **Award type:** 5
- **Project period:** 1978-05-01 → 1989-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3125960

## Citation

> US National Institutes of Health, RePORTER application 3125960, IMMUNITY MECHANISMS IN HERPESVIRUS INFECTIONS (5R01AI014981-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3125960. Licensed CC0.

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