# CONTROL OF JUVENILE HORMONE SYNTHESIS

> **NIH NIH R01** · UNIVERSITY OF IOWA · 1990 · $127,972

## Abstract

The long term goal is to understand the regulation of synthesis of juvenile
hormone (JH), a major insect hormones involved in the control of
reproduction.  JH is synthesized by a pair of glands, the corpora allata
(CA).

The system studied is the reproductive cycle of the cockroach, Diploptera
punctata, in which both the brain and the ovary interact to regulate the
changes in JH synthesis required for egg development.  This system serves
as a model for investigating the regulation of hormone synthesis and should
provide basic information which will be useful in development methods of
controlling insect reproduction.

The specific aims of the proposal are, first, to study biological
parameters of D. punctata allatostatins, neuropeptide inhibitors of JH
synthesis, that have recently been isolated, sequenced and synthesized.
This will include the study of their localization and quantitation in the
nervous system and hemolymph, their effects on CA of different
physiological states, their binding to CA cells and effect on second
messengers, and an investigation of the factors that elicit their release.
A second goal is to isolate other allatostatins and allatotropins from the
brain and ovary.

Antibodies will be prepared to the synthetic allatostatins for
immunocytochemical localization by light and electron microscopy and for
quantitation of the allatostatins by enzyme-linked immunosorbent assay.  JH
synthesis, as determined by a direct short term in vitro radiochemical
assay, will be used to measure the effect of allatostatins or extracts in
which allatostatins and allatotropins are sought.  Extracts will be
purified by high-pressure liquid chromatography.

## Key facts

- **NIH application ID:** 3126059
- **Project number:** 2R01AI015230-11
- **Recipient organization:** UNIVERSITY OF IOWA
- **SAM.gov UEI:** Z1H9VJS8NG16
- **Principal Investigator:** BARBARA A STAY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $127,972
- **Award type:** 2
- **Project period:** 1979-05-01 → 1995-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126059

## Citation

> US National Institutes of Health, RePORTER application 3126059, CONTROL OF JUVENILE HORMONE SYNTHESIS (2R01AI015230-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126059. Licensed CC0.

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