# COORDINATION OF E COLI RIBOSOMAL PROTEIN SYNTHESIS

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1993 · $343,031

## Abstract

The synthesis of ribosomes is regulated in response to environment and
development.  A well-documented example of this is the growth rate
control of ribosome synthesis in Escherichia coli.  The goal of this
project is to unravel the molecular mechanisms underlying this
regulation.  The information gained by this study should contribute to
tbe understanding of regulation of cell growth.  It will also elucidate
mechanistic aspects of the processes of transcription termination and
translation initiation.

The E. coli ribosomal protein L-4, encoded by the third gene of the
eleven gene S10 operon, is a multifunctional protein which serves as a
ribosomal component and as a regulator of both transcription termination
in the S10 leader and translation of the first gene of the operon.  The
purpose of the experiments proposed here is to provide a detailed
molecular model for the two regulatory processes induced by excess L4.
Currently it is known that transcription termination requires L4, NusA
and certain leader sequences, but it is not clear how these components
interact to prompt the RNA polymerase to terminate transcription at a
specific site.  Furthermore, it appears likely that additional
component(s) are necessary for completion of the terrmination process.
The mechanism for the translation control has not yet been elucidated.
A proposed role of alternative secondary RNA structures will be tested
and in vitro systems will be established for biochemical analysis of the
translation regulation.  Finally, L4 mutants will be analyzed to
identify domains involved in the protein's various functions.

Other experiments will explore new features of the regulation of the S10
operon: (i) possible upstream activating sequences, (ii) possible
involvement of sequences outside of the promoter region in steady state
growth control, and (iii) expression during stationary phase and out
growth.

## Key facts

- **NIH application ID:** 3126143
- **Project number:** 5R01AI015286-16
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** LASSE A LINDAHL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $343,031
- **Award type:** 5
- **Project period:** 1978-07-01 → 1996-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126143

## Citation

> US National Institutes of Health, RePORTER application 3126143, COORDINATION OF E COLI RIBOSOMAL PROTEIN SYNTHESIS (5R01AI015286-16). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126143. Licensed CC0.

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