# ORIGIN AND DIFFERENTIATION OF MONOCYTES

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 1988 · $133,245

## Abstract

Mononuclear phagocytes including monocytes play a major role in
inflammation, infection and various immunologic reactions.  Bone marrow is
considered the main source of circulating monocytes.  In this study, we
plan to isolate and characterize different classes of monocyte precursors
in bone marrow.   This will allow us to study the factors that control and
regulate the supply of monocytes and macrophages under normal steady state
and pathological conditions.  Three classes of cells belonging to monocyte
lineage have been morphologically identified.  They are bone marrow
monocyte, promonocyte and monoblasts.  However, cells less differentiated
than monoblasts are not well characterized.  Recent studies have shown that
about 4-6% of nucleated bone marrow cells bind to a class of
colony-stimulating factor specific for mouse mononuclear phagocytes
(CSF-1).  We will produce a monoclonal antibody against CSF-1.  A rat is
hyperimmunized with purified CSF-1.  The spleen cells are then fused with
mouse myeloma cells.  Monoclonal antibody specific for CSF-1 is selected
and used for subsequent studies.  Monocyte precursors in bone marrow are
first isolated by indirect immunofluorescent technique using anti-CSF-1
antibody as the first antibody and fluorescent-activated cell sorter.
These CSF-1-positive cells are then analyzed according to their size,
morphology, enzymatic activity the presence of receptors (FcR, CR and CR2)
and ability to replicate in tissue culture.  We will also develop
monoclonal antibodies reactive against blood monocytes and other
mononuclear phagocytes.  These antibodies will be used to isolate monocyte
precursors and study their surface properties.

## Key facts

- **NIH application ID:** 3126241
- **Project number:** 5R01AI015542-08
- **Recipient organization:** WASHINGTON UNIVERSITY
- **SAM.gov UEI:** L6NFUM28LQM5
- **Principal Investigator:** HSIU-SAN LIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $133,245
- **Award type:** 5
- **Project period:** 1980-03-01 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126241

## Citation

> US National Institutes of Health, RePORTER application 3126241, ORIGIN AND DIFFERENTIATION OF MONOCYTES (5R01AI015542-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126241. Licensed CC0.

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