# VIRAL INFECTION AND CELL-MEDIATED IMMUNITY

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1988 · $128,001

## Abstract

Influenza virus infection continues to be associated with substantial
morbidity and mortality, and varied effects on human immune responses.
Interferon production is stimulated but lymphocyte transformation responses
are depressed.  The specific aims of the proposed research are to determine
(1) whether depressed transformation responses are due to
macrophage-mediated suppression or macrophage dysfunction; (2) whether
infected cells elicit a different spectrum of response than the infectious
virus; and (4) in order to accomplish these aims, to identify the
population(s) of human mononuclear leukocytes expressing viral antigens.
(This work is a continuation of studies instituted under a Young
Investigator Research Grant, AI 15547).  Suppression of lymphobyte
transformation by cells or soluble mediators will be tested by coculturing
of control and virus-exposed cells, by addition to control cultures of
supernatant culture fluids from virus-exposed cells, and by use of
inhibitors of prostaglandin and hydrogen peroxide synthesis.  Populations
will be identified and sorted into infected and uninfected populations
using flow cytometry and indirect immunofluorescent labelling with goat
anti-influenza heteroantisera and mouse monoclonal anti-influenza
antibodies.  Monoclonal antibodies to leukocyte markers will be used to
identify leukocyte subsets.  Cells expressing viral antigens will be
compared for function (such as support of transformation responses,
stimulation of interferon production, stimulation of an autologous mixed
leukocyte reaction) with non-infected cells.  Understanding the mechanisms
of the virus-induced effects on human immune function may aid in
development of improved preventive or therapeutic regimens, and perhaps in
the development of diagnostic assays for viral infection.

## Key facts

- **NIH application ID:** 3126248
- **Project number:** 5R01AI015547-09
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** Norbert J. Roberts
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $128,001
- **Award type:** 5
- **Project period:** 1979-01-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126248

## Citation

> US National Institutes of Health, RePORTER application 3126248, VIRAL INFECTION AND CELL-MEDIATED IMMUNITY (5R01AI015547-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126248. Licensed CC0.

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