# CYTOTOXIC T LYMPHOCYTE RESPONSE TO INFLUENZA VIRUS

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 1990 · $202,192

## Abstract

This proposal is designed to investigate viral antigen recognition by H-2
restricted Cytolytic T Lymphocytes (CTL) and the process of viral antigen
presentation in target cell sensitization for CIL recognition.  It is a
continuation of our ongoing study of the CIL response to type A influenza
virus in the mouse.  An important new direction in our research program has
come from recent studies on a newly defined population of virus-specific
CTL in the mouse.  In addition to conventional CTL restricted in viral
antigen recognition by class I (H-2K/D) major histocompatibility (MHC)
locus products there is now good evidence for the existence of anti viral
CTL restricted by class II (H-2I region) major histocompatibility locus
products.  The characterization of these class I  MHC-restricted CTL as
well as the continuing study of class II MHC restricted CTL is the focus of
this research program.  Our experimental approach will primarily employ
clonal populations of H-2K/D and H-2I region restricted CTL:  i) to define
the influenza viral gene products which can serve as CTL target antigens;
ii) to probe the molecular forms of viral antigen which can render target
cells susceptible to lysis by CTL; iii) to map antigenic epitopes on the
influenza hemagglutinin molecule recognized by CTL.  Several eukaryotic
expression vectors will be employed to get expression of influenza genes in
various target cells and to get expression of truncated genes.  This
investigation should provide basic information on the immune response to an
important human pathogen.  Furthermore, these studies should help to
elucidate the similarities and differences in the forms of viral antigens
recognized by these two distinct CTL subsets and thereby provide a
framework for future vaccine design.

## Key facts

- **NIH application ID:** 3126275
- **Project number:** 5R01AI015608-11
- **Recipient organization:** WASHINGTON UNIVERSITY
- **SAM.gov UEI:** L6NFUM28LQM5
- **Principal Investigator:** Thomas J Braciale
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $202,192
- **Award type:** 5
- **Project period:** 1979-09-01 → 1991-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126275

## Citation

> US National Institutes of Health, RePORTER application 3126275, CYTOTOXIC T LYMPHOCYTE RESPONSE TO INFLUENZA VIRUS (5R01AI015608-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126275. Licensed CC0.

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