# CHEMICAL & SEROLOGICAL STUDIES OF LYMPHOCYTE SURFACES

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1987 · $151,048

## Abstract

The first aim of this research is to examine the primary structure of
several T cell antigens which are similar in overall structure to the
HLA-A, -B, -C antigens and which may be the human homologues of the murine
TL and Qa antigens.  The problem will first be approached by purifying each
of the antigens by sequential lectin and monoclonal antibody affinity
chromatography.  Monoclonal antibodies that react with two of these
"HLA-like" antigens are available.  Using partially purified material as
immunogen, monoclonal antibodies will be generated to a
Beta2-microglobulin-associated cell surface antigen(s) and to a
Beta2-microglobulin-like protein (Betat) for which there are currently no
monoclonal antibodies available.  The purified molecules will be
characterized structurally.  The purified antigen will also be employed in
functional studies.  Such studies may be useful in determining the
evolutionary origin of these molecules and may lead to insights into their
functional roles.  The second aim is to examine the primary structure of
some human T cell specific proteins which are present on a human cytotoxic
T cell line which specifically recognizes an allogeneic HLA-DR antigen.
This problem will be approached by first constructing a cDNA library from
the T cell line and then from this library obtaining a sub-library
containing only clones which are specific for T cells.  These clones will
then be correlated with recently identified T cell specific proteins by
hybrid selection of mRNA, cell-free translation and immunoprecipitation.
Finally, when cDNA clones have been correlated with specific proteins, then
DNA sequencing will be carried out in order to obtain amino acid sequence
information about the proteins of interest.  It is anticipated that one or
more of these proteins may be components of the T cell recognition unit ("T
cell receptor") for the HLA-DR alloantigen.

## Key facts

- **NIH application ID:** 3126341
- **Project number:** 5R01AI015669-10
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** JACK L STROMINGER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $151,048
- **Award type:** 5
- **Project period:** 1978-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126341

## Citation

> US National Institutes of Health, RePORTER application 3126341, CHEMICAL & SEROLOGICAL STUDIES OF LYMPHOCYTE SURFACES (5R01AI015669-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126341. Licensed CC0.

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